TABLE 1.
Strains and plasmids used
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| Strains | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 deoC1 ptsF25 rpsR flbB301 | 10 |
| BB1553 | MC4100 ΔdnaK52 sidB1 | 8 |
| GW8301 | MC4100/pJM100 | 29 |
| GW8302 | BB1553/pJM100 | 29 |
| GW8303 | GW8301/pBR322 | 29 |
| GW8305 | GW8301/pJM41 | 29 |
| GW8306 | GW8302/pJM41 | 29 |
| GW8309 | GW8301/pJM43 | 29 |
| TB1009 | GW8301/pTB204 | This work |
| TB1031 | GW8301/pTB107 | This work |
| JC7623 | recB recC sbcB | 26 |
| NA7623 | JC7623 ndk::km | 26 |
| TB3200 | NA7623 ΔdnaK52 | This work |
| TB3500 | JC7623 ΔdnaK52 | This work |
| TB3220 | TB3200/pJM6 | This work |
| TB3520 | TB3500/pJM6 | This work |
| TB3221 | TB3200/pTB127 | This work |
| TB3222 | TB3200/pJM8 | This work |
| TB3223 | TB3200/pTB124 | This work |
| Plasmids | ||
| pBS | Stratagene | |
| pBR322 | New England Biolabs | |
| pJM100 | pACYC177 KmrlacIq | 29 |
| pJM41 | pBR322-PlacdnaK+dnaJ+ | 29 |
| pJM43 | pBR322-PlacdnaK(T199A) dnaJ+ | 29 |
| pTB204 | pBR322-PlacdnaK(T199S) dnaJ+ | This work |
| pTB107 | pBR322-PlacdnaK(K70A) dnaJ+ | This work |
| pJM6 | pBS-PlacdnaK+ | 28 |
| pJM8 | pBS-PlacdnaK(T199A) | 28 |
| pTB124 | pBS-PlacdnaK(T199S) | This work |
| pTB127 | pBS-PlacdnaK(K70A) | This work |