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. 2022 Apr 18;3:835776. doi: 10.3389/falgy.2022.835776

Figure 2.

Figure 2

FcεRI crosslinking induces miR-155-5p expression in human and mouse mast cells. Human SMCs isolated from tissues of different donors (A, n = 3) and mouse BMMCs from male C57BL6 mice (B, n = 3) were sensitized with anti-NP IgE or anti-DNP IgE, respectively, and activated for 3 h at 37°C with 1–100 ng/ml of the multivalent antigen NP-BSA or DNP-HSA. Changes in miR-155-5p expression were determined by quantitative RT-PCR using the 2ΔΔCq method with SNORD96A as the internal reference control. The bar graphs represent mean ± SEM of values obtained from independent experiments with three different BMMC or SMC cultures from different mice or human donors. Significance was determined with Student's t-test. *p < 0.05; ***p < 0.001.