Figure 3.

Genotyping and characterization of miR-155 knockout (KO) BMMCs. (A) Representative DNA gel of PCR products. Genotyping was routinely performed on all BMMC cultures to confirm miR-155 deficiency. (B) Quantitative RT-PCR analysis of miR-155-5p expression in wild-type and miR-155 KO BMMCs. (C) Representative flow cytometry histogram of surface FcεRI expression on wild-type and miR-155 KO BMMCs. (D) Bar graph showing relative amount of total β-hexosaminidase in miR-155 BMMCs compared to wild-type BMMCs. The bar graphs represent mean ± SEM of values obtained from independent experiments with three different BMMC cultures from different mice. Significance was determined with Student's t-test.