Figure 5.

FcεRI-induced cytokine production is impaired in miR-155 KO BMMCs. Wild-type and miR-155 KO BMMCs were sensitized with anti-DNP-IgE and challenged with multivalent antigen DNP-HSA for 24 h. Secreted TNF (A), IL-6 (B), and IL-13 (C) in supernatant were measured by commercial ELISA. The bar graphs represent mean ± SEM of values obtained from independent experiments with three different BMMC cultures from different mice. Cytokines in untreated samples were generally below the level of detection. Lowest level of detection for TNF, IL-6, and IL-13, respectively, were 31.2, 15.6, and 62.5 pg/ml. n.d., not detected. Significance was determined with Student's t-test. **p < 0.01; ***p < 0.001.