Figure 7.

FcεRI-induced Akt phosphorylation is specifically inhibited in miR-155 KO BMMCs. Representative Quantitative Western blot showing phosphorylation status of Akt, p38, and p44/42 in wild-type and miR-155 KO BMMCs following FcεRI crosslinking with multivalent antigen. IgE-sensitized BMMCs were activated for 5 min with NP-BSA (10 ng/ml) and whole cell lysates were separated by reducing SDS-PAGE. Total and phosphorylated proteins were analyzed on the same blot by 2-color (680 and 800 nm) multiplexing on a near-infrared detection system. Blot is representative of at least three independent experiments with different BMMC cultures from different mice. Fold change in phosphorylation was calculated relative to non-activated wild-type BMMCs.