TABLE 2.
Nalidixic acid responses on solida and in liquid media
| Fusion to gene or operon | Lux identification | Solid response ratio at:
|
Liquid maximal response concn (μg/ml) | Liquid response ratio at 2 h | Liquid concn range >1.5-fold upb (μg/ml) | |||
|---|---|---|---|---|---|---|---|---|
| 2 h | 4 h | 6 h | 8 h | |||||
| Known SOS regulon members | ||||||||
| ydjM | lux-a.pk033.c5 | 3.7 | 4.3 | 3.2 | 2.6 | 5 | 3.7 | 1.2–80 |
| 2.1 | 3.1 | 3.3 | 2.5 | |||||
| dinG ybiB | lux-a.pk024.f5 | 3.4 | 4.2 | 3.1 | 2.6 | 5 | 4.2 | 1.2–40 |
| 2.5 | 3.7 | 3.5 | 2.9 | |||||
| dinB | lux-a.pk055.a3 | 3.1 | 5.1 | 4.2 | 3.1 | 5 | 7.6 | 1.2–80 |
| 5.5 | 11.0 | 8.1 | 6.3 | |||||
| uvrA | lux-a.pk001.b6 | 2.5 | 2.3 | 1.6 | 1.0 | ndc | nd | nd |
| 2.9 | 2.4 | 1.1 | 0.6 | |||||
| Nalidixic acid upregulated on solid LuxArray and in liquid culture | ||||||||
| ycgH | lux-a.pk015.d6 | 2.3 | 6.4 | 2.7 | 0.6 | 20 | 2.7 | 1.2–40 |
| 2.2 | 4.1 | 1.9 | 0.8 | |||||
| intG | lux-a.pk019.g1 | 2.5 | 7.7 | 9.7 | 7.1 | 10 | 2.3 | 1.2–40 |
| 4.4 | 8.2 | 15.5 | 10.2 | |||||
| lpxA lpxB rnhB dnaE | lux-a.pk061.c3 | 3.5 | 3.1 | 2.8 | 1.7 | 2.5 | 1.8 | 1.2–5.0 |
| 2.7 | 3.5 | 3.0 | 1.9 | |||||
| oraA | lux-a.pk058.f5 | 5.1 | 7.9 | 5.9 | 3.9 | 10 | 7.2 | 1.2–80 |
| 4.7 | 7.7 | 5.6 | 3.8 | |||||
| rihC | lux-a.pk031.e7 | 2.1 | 2.0 | 1.3 | 1.0 | 2.5 | 1.9 | 1.2–5.0 |
| 2.0 | 2.1 | 1.8 | 1.5 | |||||
| yigN | lux-a.pk046.f11 | 2.1 | 2.5 | 0.3 | 0.1 | 1.2 | 2.5 | 1.2–20 |
| 7.1 | 4.3 | 2.5 | 2.2 | |||||
| Nalidixic acid upregulation not reproduced in liquid culture | ||||||||
| frvR frvX frvB frvA | lux-a.pk046.e6 | 2.8 | 2.3 | 0.8 | 0.5 | 20 | 1.5 | None |
| 2.1 | 2.3 | 2.0 | 1.7 | |||||
| yfhJ fdx hscA yfhE | lux-a.pk0019.g2 | 2.1 | 2.0 | 1.7 | 1.4 | 1.2 | 1.0 | None |
| 2.1 | 2.2 | 1.5 | 1.3 | |||||
a
The responses in the solid LuxArray experiment are given for each of the two independent spots.
b
Nalidixic acid concentration ranges in cultures with response ratios of 1.5 or greater.
c
nd, not done..