FIG. 1.

Effects of overexpression and disruption of kbpA in S. coelicolor A3(2) M130. (A) Strain M130 harboring pIJ6021 produces the blue pigment actinorhodin, whereas that harboring kbpA on pIJ6021 produces a much smaller amount of the pigment. (B) Schematic representation of the chromosomal kbpA disruption. The kbpA coding region is completely deleted so that this deletion does not affect the promoter in front of kbpA or afsK. Abbreviations: Ec, EcoRI; Not, NotI; Sp, SphI; Xb, XbaI. (C) The kbpA disruptant produces a larger amount of actinorhodin than strain M130.