TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Genotype or description | Source (reference) |
|---|---|---|
| Strains | ||
| E. coli DH5α | Φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 hsdR17(rK− mK+) supE44 relA1 deoR Δ(lacZYA-argF)U169 | Laboratory stock |
| B. subtilis | ||
| DB104 | his nprR2 nprE18 ΔaprA3 | Laboratory stock |
| DB104ΔpyrR | HC11 pyrB+ Specs ΔpyrR | Tumer et al. (33) |
| DB104ΔpyrDII | DB104 ΔpyrDII | Kahler and Switzer (14) |
| HC11 | DB104 pyrB::Specr | Hu and Switzer (11) |
| DB104ΔpyrDIIcdd | DB104 ΔpyrDII cdd::pMX | This study |
| DB104pyrG::cm | DB104 pyrG::Cmr | This study |
| DB104pyrG::erm | DB104 pyrG::Ermr | This study |
| QM201 | DB104 amyE::pMSAF | This study |
| QM202 | DB104 ΔpyrR amyE::pMSAF | This study |
| QM203 | DB104 ΔpyrDII amyE::pMSAF | This study |
| QM204 | DB104 ΔpyrDIIcdd amyE::pMSAF | This study |
| QM205 | HC11 amyE::pMSAF | This study |
| QM206 | DB104 pyrG::erm amyE::pMSAF | This study |
| QM102 | HC11 amyE::pMSAD | This study |
| QM103 | HC11 amyE::pMSAH | This study |
| QM104 | HC11 amyE::pMSAB | This study |
| QM105 | HC11 amyE::pMSCD | This study |
| QM234 | DB104 ΔpyrDIIcdd amyE::pMSAB | This study |
| QM301 | HC11 amyE::pMSANMF | This study |
| Plasmids | ||
| pUC19 | Apr | Yanisch-Perron et al. (38) |
| pDH32 | Apr CmramyE front amyE back lacZ | Grandoni et al. (8) |
| pFL32 | AprrpoE gene in XbaI-HindIII sites of pUC19 | López de Saro et al. (17) |
| pMutin | Apr Emr | Vagner et al. (34) |
| pJH4133 | Cmr, contains pyrG gene | Trach et al. (32) |
| pJH4521 | Apr Cmr, pyrG gene with Cm insertion | Trach et al. (32) |
| pCm::Er | Apr Emr | Steinmetz and Richter (30) |
| pHV502 | pMutin with a 2.4-kb BclI deletion | This study |
| pMX | 186 bp of HindIII-BamHI fragment of cdd gene inserted into HindIII-BamHI-digested pHV502 | This study |
| pUCAN | EcoRI (−96)-SacI (+20) PCR fragment inserted into pUC19 digested with EcoRI and SacI | This study |
| pUCANMF | SacI (+30)-BamHI (+218) PCR fragment inserted into pUCAN digested with SacI and BamHI | This study |
| Integration plasmids | ||
| pMSAF | EcoRI (−96)-BamHI (+218) PCR fragment inserted into pDH32 digested with EcoRI and BamHI | This study |
| pMSAB | EcoRI (−96)-BamHI (+36) PCR fragment inserted into pDH32 digested with EcoRI and BamHI | This study |
| pMSCD | EcoRI (+57)-BamHI (+110) PCR fragment inserted into pDH32 digested with EcoRI and BamHI | This study |
| pMSAD | EcoRI (−96)-BamHI (+110) PCR fragment inserted into pDH32 digested with EcoRI and BamHI | This study |
| pMSAH | EcoRI (−96)-BamHI (+80) PCR fragment inserted into pDH32 digested with EcoRI and BamHI | This study |
| pMSANMF | 0.32-kb EcoRI-BamHI fragment of pUCANMF in pDH32 | This study |