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. 2022 Jul 19;119(10):2919–2937. doi: 10.1002/bit.28169

Figure 1.

Figure 1

Production of HIV envelope (Env) gp140 in plants with improved glycosylation. (a) Overlayed normalized size exclusion chromatography elution profiles of gp140 when produced in human embryonic kidney 293 (HEK293) cells or in plants with modified (glycan‐enhanced [GE]) or unmodified glycosylation (wild type [WT]). The peaks corresponding to aggregates and putative trimers are indicated by the cartoon image above the figure. (b) Coomassie‐stained blue native polyacrylamide gel electrophoresis (BN‐PAGE) gel comprising of size exclusion chromatography (SEC)‐fractionated material from all three protein variants. The number above each lane refer to a corresponding fraction from size exclusion chromatography. Top: WT; middle: GE; bottom: HEK293. (c) Western blot analysis of the pooled fractions following SEC. HIV Env was detected using polyclonal goat anti‐gp120 antibody.