Figure 3.

Comparison of the glycan occupancy of recombinant HIV envelope (Env) gp140 when produced in N. benthamiana using integrated host and transient glyco‐engineering (“glycan‐enhanced”) or no glyco‐engineering expression approaches (wild type [WT]). (a) The percentage point changes in glycan occupancy are labeled onto the model generated in Figure 2. A positive change represents a glycan site that is more occupied on the “glycan‐enhanced” material compared with the WT. (b) Site‐specific glycan occupancy of recombinant HIV Env gp140 produced in WT and “glycan‐enhanced” N. benthamiana. The change in glycan occupancy is colored, with red representing a decrease and blue an increase.