TABLE 3.
Activation of qsc102, qsc117, and qsc102 8A-13T promoter-lacZ fusions by the las and rhl systems in E. coli
| Activatorb | β-Galactosidase activitya ± SEM of:
|
||
|---|---|---|---|
| qsc102 | qsc117 | qsc102 8A-13T | |
| LasR | 4 ± 1 | 35 ± 5 | 12 ± 3 |
| LasR + 3OC12-HSL | 140 ± 5 | 107 ± 7 | 128 ± 2 |
| Vector + 3OC12-HSL | 2 ± 1 | 15 ± 2 | 20 ± 2 |
| RhlR | 3 ± 1 | 30 ± 4 | 9 ± 1 |
| RhlR + C4-HSL | 4 ± 1 | 132 ± 5 | 97 ± 2 |
| Vector + C4-HSL | 2 ± 2 | 16 ± 5 | 21 ± 2 |
a
Expressed as relative light units/OD660.
b
The control vector was pACYC184, and lasR and rhlR were supplied on pPCS11 and pECP11, respectively.