TABLE 4.
Activation of lasI and rsaL in a P. aeruginosa lasI rhlI double mutant by addition of acyl-HSL
| Fusion (plasmid) | β-Galactosidase activity (Miller units)a ± SEM with:
|
||
|---|---|---|---|
| No addition | 3OC12-HSL | 3OC12-HSL + C4-HSL | |
| lasI-lacZ (pMW313) | 5 ± 1 | 19 ± 1 (4-fold) | 18 ± 1 (4-fold) |
| rsaL-lacZ (pMW312) | 231 ± 31 | 5,668 ± 165 (25-fold) | 5,755 ± 546 (25-fold) |
a
β-Galactosidase activities of the lasI-lacZ and rsaL-lacZ fusions were 17 and 5,272 miller units, respectively, in wild-type P. aeruginosa PAO1. Values in parentheses are fold increases over activity with no addition.