Life history parameters of backcrossed wMel, wMelM and wMel.tet Aedes aegypti populations. The wMel (blue), wMelM (red) and wMel.tet (black) populations were evaluated for the following traits: larval development time for (A) females and (B) males, longevity of (C) females and (D) males, (E) female fecundity across gonotrophic cycles, (F) egg hatch proportion across gonotrophic cycles, and wing length of (G) females and (H) males. Each point represents data averaged across a replicate container of 100 individuals (A and B) or data from individual mosquitoes (panels E–H). For each treatment/strain, we measured 12 replicate trays for development time (A–B), 8 replicate cages of 25 females and 25 males for longevity (C and D), 50 individual females for fecundity and egg hatch (E and F) and 20 individuals for wing length (G and H). Medians and 95% confidence intervals are shown in lines and error bars.