Table 2.
Bacterial strains and plasmids used in this study.
| Strain or plasmid | Relevant properties a | Reference |
|---|---|---|
| E. coli | ||
| TOP10 | Invitrogen | |
| L. pneumophila | ||
| AK01 (ΔlqsT) | JR32 lqsT::Km | Kessler et al. (2013) |
| AK02 (ΔlqsS‐ΔlqsT) | JR32 lqsS::Km lqsT::Gm | Kessler et al. (2013) |
| AK03 (ΔlvbR) | JR32 lvbR::Km | Hochstrasser et al. (2019) |
| ER01 (ΔlegG1) | JR32 legG1::Km | Rothmeier et al. (2013) |
| GS3011 (ΔicmT) | JR32 icmT3011::Km | Segal and Shuman (1998) |
| JR32 | L. pneumophila Philadelphia‐1, serogroup 1, salt‐sensitive isolate of AM511 | Sadosky et al. (1993) |
| LS01 (ΔppgA‐ΔlegG1) | JR32 ppgA::Gm legG1::Km | Swart et al. (2020b) |
| LS03 (ΔppgA) | JR32 ppgA::Km | Swart et al. (2020b) |
| NT02 (ΔlqsA) | JR32 lqsA::Km | Tiaden et al. (2010b) |
| NT03 (ΔlqsR) | JR32 lqsR::Km | Tiaden et al. (2007) |
| NT05 (ΔlqsS) | JR32 lqsS::Km | Tiaden et al. (2010b) |
| ΔflaA | JR32 flaA::Km | Weber et al. (2012) |
| Plasmids | ||
| pAK18 | pMMB207C‐P lvbR ‐lvbR, gfp (constitutive) | Hochstrasser et al. (2019) |
| pCM009 | pMMB207C‐P flaA ‐gfp (ASV) | Schell et al. (2016) |
| pNP102 | pMMB207C, ΔlacI q, mcherry (constitutive) | Steiner et al. (2017) |
| pNT28 | pMMB207C, gfp (constitutive) | Tiaden et al. (2007) |
| pNT31 | pMMB207C‐P lqsS ‐lqsS, gfp (constitutive) | Tiaden et al. (2010b) |
| pRH022 | pMMB207C‐P lvbR ‐lvbR‐P flaA ‐gfp (ASV) | This study |
| pRH031 | pMMB207C‐P csrA ‐gfp (ASV), Cm | This study |
| pRH032 | pMMB207C‐P ralF ‐gfp (ASV), Cm | Striednig et al. (2021) |
| pRH035 | pMMB207C‐P sidC ‐gfp (ASV), Cm | Striednig et al. (2021) |
| pRH046 | pMMB207C‐P csrA ‐gfp (AAV), mcherry (constitutive), Cm | This study |
| pRH047 | pMMB207C‐P ralF ‐gfp (AAV), mcherry (constitutive), Cm | This study |
| pRH049 | pMMB207C‐P 6SRNA ‐gfp (ASV), Cm | This study |
| pRH050 | pMMB207C‐P sidC ‐gfp (AAV), mcherry (constitutive), Cm | This study |
| pSB001 | pMMB207C‐P flaA ‐flaA, gfp (constitutive) | This study |
| pSN7 | pMMB207C‐P flaA ‐gfp (AAV), mcherry (constitutive), Cm | Personnic et al. (2019) |
| pSN25 | pMMB207C‐P 6SRNA ‐gfp (AAV), mcherry (constitutive), Cm | Personnic et al. (2021) |
a
Abbreviations: Cm, chloramphenicol resistance; Km, kanamycin resistance; Gm, gentamicin resistance.