TABLE 4.
Mean and standard deviation (in parenthesis) ProtoCol 3 density values from plates with Escherichia coli and Staphylococcus aureus which were fixed with different concentration of glutaraldehyde for either 2 or 5 min
| Glutaraldehyde concentration (%) | Fixation time (min) | p‐values | |||
|---|---|---|---|---|---|
| 2 | 5 | ||||
| Post PBS wash | 2.5 | 81 (2) | 79 (1) | Concentration | 0.325 |
| 1.0 | 80 (5) | 79 (3) | Fixation time | 0.325 | |
| Interaction | 0.999 | ||||
| After 24 h | 2.5 | 38 (2) | 29 (4) | Concentration | 0.380 |
| 1.0 | 38 (2) | 31 (6) | Fixation time | <0.001 | |
| Interaction | 0.380 | ||||
Note: Measurements were taken immediately after the third PBS wash and after 24 h. All values were then expressed as a percentage of the pre‐fixation density, n = 3. Statistical comparisons were made using two‐way ANOVA with replication.
Abbreviations: ANOVA, analysis of variance; PBS, phosphate‐buffered saline.