TABLE 5.
Mean and standard deviation (in parenthesis) ProtoCol 3 density values from plates with Serratia marcescens and Micrococcus luteus which were fixed with different concentration of glutaraldehyde for either 2 or 5 min
| Glutaraldehyde concentration (%) | Fixation time (min) | p‐values | |||
|---|---|---|---|---|---|
| 2 | 5 | ||||
| Post PBS wash | 2.5 % | 117 (3) | 104 (10) | Concentration | 0.387 |
| 1.0 % | 100 (5) | 117 (8) | Fixation time | 0.397 | |
| Interaction | <0.001 | ||||
| After 24 h | 2.5 % | 92 (4) | 102 (7) | Concentration | <0.001 |
| 1.0 | Fixation time | <0.001 | |||
| 1.0 % | 83 (3) | 102 (8) | Interaction | <0.05 | |
Note: Measurements were taken immediately after the third PBS wash and after 24 h. All values were then expressed as a percentage of the pre‐fixation density, n = 3. Statistical comparisons were made using two‐way ANOVA with replication.
Abbreviations: ANOVA, analysis of variance; PBS, phosphate‐buffered saline.