TABLE 2.
Plasmids
| Plasmid | Relevant characteristic(s) | Source |
|---|---|---|
| pNK2883 | Ampr, inducible mini-Tn10 with isopropyl-β-d-thiogalactopyranoside promoter | Kleckner et al. (18) |
| pSP72 | High copy number, Ampr | Promega |
| pHSG576 | Low copy number, Cmr, pSC101 derivative | Takeshita et al. (31) |
| pSM1 | pSP72 with a modified multiple cloning site replacing the pSP72 sites between the EcoRI and HindIII sites with the following restriction sites: NotI, SfiI, BamHI, NotI, and SfiI | This study |
| pSM2 | pHSG576 with a modified multiple cloning site replacing the pHSG576 sites between the EcoRI and HindIII sites with the following restriction sites: NotI, SfiI, BamHI, NotI, and SfiI | This study |
| pSM3 | pSP72 with msbB+ | This study |
| pSM21 | pSM2 with msbB+ | This study |
| pSM22 | pSM2 with somA+ | This study |