Amino acid exchanges in the mutant PrfA proteins obtained from the group II transformants of B. subtilis DB104 carrying pLSV18 and pERL3502. Upper lane, amino acid sequence encoded by wild-type prfA; the amino acids below indicate the altered positions in the various group II mutants. HTH, helix-turn-helix motif; arrowheads, putative leucine zipper motifs; asterisk, stop codon; -, deleted amino acids. Each marked amino acid alteration belongs to an individual mutant.