FIG. 4.

(A) EMSA with a ³²P-labeled hly promoter DNA fragment and protein extracts of B. subtilis DB104 transformants carrying the various mutated prfA genes (see Fig. 3). As controls protein extracts of L. monocytogenes EGD (C+), L. monocytogenes prfA mutant Δ42 (C−), and B. subtilis DB104 carrying pERL3502 (BS) were used. (B) EMSA with protein extracts from L. monocytogenes Δ42 with mutant PrfA proteins M58I, Y62C, and Y83S. CI and CII, complexes of target DNA with PrfA and RNAP (CI) or with target DNA and RNAP alone (CII) (11).