FIGURE 3.

AMPK activity was inhibited by increased ATP synthesis in AML cells co‐cultured with HS‐5 cells. (A) Representative western blot analysis of proteins involved in the AMPK‐mTORC1 pathway (total AMPK, p‐AMPK^T172, total p70S6K, p‐p70S6K^T389, total 4E‐BP1, p‐4E‐BP1^S65, and c‐Myc; β‐actin was used as loading control) from AML cells cultured alone or with HS‐5 cells. The expression levels of p‐p70S6K^T389 and p‐4E‐BP1^S65 were used as indicators of mTORC1 activity. (B) Representative western blot analysis of AMPK‐mTORC1 pathway proteins of AML cells after treatment with 10 μM Oligomycin A. The representative images of 3 independent experiments were used