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Reaction mechanism of PPA. (1) Nucleophilic attack of carboxylate group of Asp197 onto C1 of the α‐(1–4) glycosidic linkage and release of the first product; (2) hydrolysis of the β‐carboxylacetal covalent intermediate; (3) Release of the second product in α‐anomeric configuration. PPA, porcine pancreatic α‐amylase
