FIG. 3.

Binding of Phabs B7 and B9 to TouRΔA. The binding of Phabs B7 and B9 and MBP (as a negative control [25]) to _6xhisTouRΔA or ovalbumin (OVA) as a specificity control antigen was determined by ELISA. Different dilutions of Phabs were incubated on ELISA plates coated with the antigens as indicated. After washing with PBS, the bound Phab was developed using anti-M13-peroxidase conjugate and the plates were read at OD₄₉₀. The data shown are relative to the maximal OD₄₉₀ obtained by Phab B7 at the higher titer employed (OD₄₉₀ of ∼2.0).