FIG. 5.

mRNA stability of cspH. (A) Measurement of the mRNA stability at 37°C. A culture of strain UK-1 (wild type) was grown to early exponential phase at 37°C, and then rifampin was immediately added to the culture to a final concentration of 200 μg/ml. RNA was extracted at the indicated times after the addition of rifampin. Also shown is the radioactivity of primer extension products, with the product at zero time set to 100%. (B) Measurement of mRNA stability at 15°C. A culture of strain UK-1 was grown to early exponential phase at 37°C, transferred to 15°C, and incubated for 1 h. Then, rifampin was added, and RNA was extracted at each time indicated.