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. 2022 May 10;111(1):304–315. doi: 10.1111/tpj.15779

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© 2022 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Characterization of PETE and DMR6 promoter activity.

(a–d) Histochemical staining for β‐glucuronidase (GUS) activity in soil‐grown transgenic Arabidopsis (at variable ages) expressing (a) PETEp‐GUS in a true leaf from a 3‐week‐old plant, (b) DMR6p‐GUS in Hpa‐infected cotyledons from 16‐day‐old seedlings at 6 dpi, (c) DMR6p‐GUS in Hpa‐infected true leaves from a 3‐week‐old plant at 6 dpi, or (d) DMR6p‐GFP in Hpa‐infected true leaves from a 3‐week‐old plant at 6 dpi.

(e, f) Plasmid maps for (e) DMR6p;PETEp and (f) 35Sp;PETEp.

(g, h) Confocal microscopy images of GFP fluorescence from 3‐week‐old transgenic Arabidopsis expressing (g) DMR6p;PETEp (at 6 dpi after infection with Hpa) and (h) 35Sp;PETEp (uninfected). [Colour figure can be viewed at wileyonlinelibrary.com]