FIG. 2.

RT-PCR of the hrpRS transcript. Total RNA was extracted using Trizol and precipitated, and 2 μg was treated with DNase I. The DNase I-treated RNA preparation was used for cDNA synthesis using the P24200 primer (Fig. 1) and for a −RT control. Genomic DNA (lanes D), the cDNA preparation (lanes C), and the −RT control (lanes −RT) were used as templates for PCR employing primers P24200 and P24634 to amplify an hrpS region (A), primers P24200 and P25591 to amplify an hrpRS fragment (B), or primers P24901 and P25591 to amplify an hrpR fragment (C). Panel D is similar to panel C except that DC3000 RNA and DC3000-specific primers were employed. Molecular sizes shown on the left in kilobases were estimated using a 1-kb ladder obtained from Invitrogen.