TABLE 3.
Activation of the hrpL promoter in E. coli MC4100 by cloned hrpR and hrpS
| MC4100 (pSGL4MS) transformant plasmid(s)a | Expressed gene(s) | Promoter activityb |
|---|---|---|
| pDSK519 | None | 1 ± 1 |
| pYXRS1D | Native hrpRS | 13,162 ± 745 |
| pSJR2L | hrpR | 1 ± 1 |
| pSJS3DS | hrpS | 83 ± 9 |
| pSJR2L, pSJS3DS | hrpR + hrpS in trans | 4,725 ± 524 |
| pSJR2S3L | Reconstructed hrpRS operon | 3,077 ± 111 |
a
The indicated plasmids are described in Table 1.
b
Promoter activity as estimated by β-galactosidase activity (in Miller units) expressed in strains carrying the indicated plasmids as described in Materials and Methods. Values are means ± standard deviations.