TABLE 4.

Interaction of HrpR and HrpS detected by yeast two-hybrid analysis

L40 transformant plasmid(s)	LexA′-BD fusion	GAL4′-AD fusion	β-Galactosidase activitya
pDWR3BTM	HrpR	Absent	<1
pDWS4BTM	HrpS	Absent	<1
pBTM-lamin, pDWR3GAD	Lamin	HrpR	<1
pBTM-lamin, pDWS4GAD	Lamin	HrpS	<1
pDWR3BTM, pGAD424	HrpR	None	<1
pDWS4BTM, pGAD424	HrpS	None	1.4 ± 0.6
pDWR3BTM, pDWR3GAD	HrpR	HrpR	<1
pDWS4BTM, pDWS4GAD	HrpS	HrpS	3 ± 0.3
pDWR3BTM, pDWS4GAD	HrpR	HrpS	29 ± 10
pDWS4BTM, pDWR3GAD	HrpS	HrpR	28 ± 10
pDWS4BTM, pTSR4GAD	HrpS	HrpRΔN	26 ± 3
pDWS4BTM, pTSR5GAD	HrpS	HrpRΔC	1.4 ± 0.5
pDWS4BTM, pTSR6GAD	HrpS	HrpRΔNΔC	1.0 ± 0.2

^aThe indicated S. cerevisiae L40 transformants were grown overnight at 25°C in defined medium lacking Leu and/or Trp (as required to maintain selection for plasmids) to an OD₆₀₀ of 1.0. Cells were harvested and lysed by three freezing cycles in liquid N₂, and β-galactosidase activity was determined as indicated in Materials and Methods.