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Absorption spectra of hemin–rHmuR-6His complex. The absorption spectra in the Soret region of rHmuR-6His (5 μM) purified and renatured from inclusion bodies alone (A) or with added hemin (20 μM) were recorded immediately (B) and 5 (C) and 15 min (D) after mixing. (E) Absorption spectrum of hemin (20 μM) added to nonrenatured rHmuR-6His (5 μM) purified from inclusion bodies and dissolved in 8 M urea (recorded after 15 min of incubation with hemin). Dotted line, 20 μM hemin alone.
