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Binding of gingipains to porphyrins and metalloporphyrins. Plates were coated with hemoglobin (Hb; 5 μM) or porphyrins or metalloporphyrins (10 μM). After incubation with RgpB (A), Kgp (B), or HRgpA (C) (0.01 to 1 nmol per well), binding was detected using antibodies to the appropriate protein. Values are representative of experiments performed in triplicate on three separate occasions. Hm, hemin.
