TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| P. gingivalis A7436 | Wild type | Laboratory collection |
| E. coli TOP10F′ | F′ {lacIq/Tn10 (Tetr)} mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 deoR araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| E. coli BL21 (DE3)pLysE | F−ompT hsdSB (rB− mB−) gal dcm (DE3)pLysE(Camr) | Invitrogen |
| E. coli BL21 (DE3)pLysS | F−ompT hsdSB (rB− mB−) gal dcm (DE3)pLysS(Camr) | Invitrogen |
| pCRT7/CT-TOPO | Ampr | Invitrogen |
| pTO1 | pCRT7/CT-TOPO containing the hmuR gene without the signal peptide sequence and with the fusion tag | 50 |
| pTO2 | pCRT7/CT-TOPO containing the hmuR gene with the signal peptide sequence and with the fusion tag | 50 |
| pTO3 | pCRT7/CT-TOPO containing the hmuR gene with the signal peptide sequence and without the fusion tag | This study |
| pTO4 | pCRT7/CT-TOPO containing the hmuR gene without the signal peptide sequence and without the fusion tag | This study |