Figure 4.

Increased full‐length Mpeg1 after BMDM activation. Day 9 BMDMs were activated overnight with LPS (10 ng mL⁻¹) and/or IFNγ (100 ng mL⁻¹). Extracts from 1 × 10⁶ cells were resolved by 10% SDS–PAGE and transferred to nitrocellulose. (a) The membranes were sequentially probed with anti‐MPEG1 antibody and rabbit anti‐GAPDH. The figure shown is representative of two independent experiments. (b, c) The fold increase of Mpeg1 on activation was evaluated via densitometry. The Student's t‐test was used to compare outcomes. *P < 0.05. BMDM, bone marrow‐derived macrophage; FL, full length; IFN, interferon; LPS, lipopolysaccharide; Mpeg1, macrophage‐expressed gene 1; P, processed form; SDS–PAGE, sodium dodecyl sulfate–polyacrylamide gel electrophoresis.