Figure 6.

Mpeg1 is not essential for bacterial clearance. WT or Mpeg1 ^−/− mice were infected with the indicated pathogens. Tissue homogenates were serially diluted and aliquots plated on appropriate media. Colonies were counted and reported as CFU/gram of tissue. (a) Six mice of each genotype infected by aerosolized Mycobacterium tuberculosis (~100–200 CFU) were killed 8 weeks after the infection and CFU in lungs and spleen assessed. Weight loss was evaluated at the indicated times after the infection. The experiment was carried out once. (b) Ten mice of each genotype were infected with 1 × 10⁷–5 × 10⁷ CFU of Staphylococcus aureus USA 300 via intranasal inhalation. At the indicated times, CFU in lungs were evaluated, and the proportions of neutrophils (Gr⁺) and macrophages (CD11b⁺, F4/80⁺) in lungs compared. The experiment was carried out three times. (c) Eight mice of each genotype were inoculated intranasally with 4 × 10⁴ CFU of Legionella longbeachae. CFU in lungs was assessed 3 days after the infection. The experiment was carried out once: each data point represents a single mouse. Statistical significance was assessed using the Student's t‐test. ns = P > 0.05. CFU, colony forming units; KO, knockout; Mpeg1, macrophage‐expressed gene 1; WT, wild type.