Figure 8.

Role of Mpeg 1 in antigen presentation. (a) H‐2K ^bm1 OCSs were intravenously injected into WT or Mpeg1 ^−/− mice, followed by intravenous injection of equal numbers of CTV^high cells labeled with ovalbumin_257–264 peptide and unlabeled CTV^low cells after 6–8 days. CTV^high and CTV^low cell numbers in recipients were determined 36–48 h later by flow cytometry. (b) Equal numbers of CTV‐labeled OT‐I T cells were intravenously injected into WT or Mpeg1 ^−/− mice, followed by intravenous injection of 20 × 10⁶ OCSs or 25 μg ovalbumin after 24 h. Dividing OT‐I T cells in spleen were enumerated 72 h later by flow cytometry. (c) Equal numbers of CTV‐labeled OT‐II T cells were intravenously injected into WT or Mpeg1 ^−/− mice, followed by intravenous injection of 20 × 10⁶ IA ^b−/− OCS mice or 50 μg ovalbumin 24 h later. About 72 h later the number of OT‐II dividing cells in spleen was assessed by flow cytometry. The experiment was performed two times. Each data point represents a single mouse. Statistical significance was assessed using the Student's t‐test. ns = P > 0.05; *P < 0.05; **P < 0.01. CTL, cytotoxic T lymphocyte; CTV, Cell Trace Violet; MHC, major histocompatibility complex; Mpeg1, macrophage‐expressed gene 1; OCS, ova‐coated splenocyte; WT, wild type.