Figure 7.

Hobit and Blimp‐1 suppress S1PR1 and CCR7 expression on Trm precursors. (A) The expression of the indicated panel of S1P receptors and chemokine receptors was determined in the tdTomato+ and tdTomato‐ fractions of virus‐specific effector CD8 T cells in the epithelial fraction of small intestine (SI IEL), kidneys, liver, and spleen of Hobit^WT/CRE mice at day 8 after infection with LCMV Armstrong using RNA sequencing. (B) S1PR1 expression was analyzed in tdTomato+ and tdTomato‐ fractions of (B) the total and (C) the virus‐specific fraction of effector CD8 T cells in kidneys of Hobit^WT/CRE mice using quantitative PCR. (D, E) The tdTomato+ fraction of CD8 T cells of SI IEL and kidney of Hobit^WT/CRE and Hobit^KO/CRE × Blimp‐1^flox/flox mice was analyzed for expression of (D) S1PR1 and (E) CCR7 using quantitative PCR. (A) Representative data of three mice per group, (B,C) five mice per group, and (E,F) three mice per group. Symbols represent individual mice. Error bars represent mean ± SEM. Unpaired t test. *p < 0.05, **p < 0.01.