FIGURE 2.

SUCNR1 activation enhances mast cell degranulation and histamine release. CD63 expression or histamine release was analyzed in CBMC treated with (A‐B) succinate or (C‐D) cES for 30 min followed by anti‐IgE challenge. Dotted lines link values from the same donors. (E) CBMC or (F‐G) LAD‐2 cells were treated with XT1 or NF‐56‐EJ40 at desired concentrations for 30 min, followed by succinate incubation and anti‐IgE challenge. (H‐I) Control and SUCNR1 KD LAD‐2 cells were incubated with succinate or cES, followed by anti‐IgE challenge. One‐way ANOVA (E‐G) with Dunnett's multiple comparison test, and RM Two‐way ANOVA with Sidak's multiple comparison test (A‐D, H‐I) were applied. *p < 0.05, **p < 0.01. ***p < 0.001. ****p < 0.0001