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. 2019 Oct 6;212(6):279–285. doi: 10.5694/mja2.50355

graphic file with name MJA2-212-279-g002.jpg

Schematic (A) and electron micrograph of bacteriophage (Myoviridae) with 1% uranyl acetate negative staining, with size marker (B). Bacteriophage preparations were dialysed against 0.1 M ammonium acetate in dialysis cassettes with a 10 000 membrane molecular weight cut‐off (Pierce Biotechnology), negatively stained with 2% uranyl acetate, and visualised using transmission electron microscopy (TEM). TEM was conducted at the Westmead Scientific Platforms (Westmead Hospital, Sydney, Australia) on a Philips CM120 BioTWIN (Thermo Fisher Scientific) transmission electron microscope at 100 kV. Images were recorded with a SIS Morada digital camera using iTEM software (Olympus Soft Imaging Solutions).