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. 2021 Jul 31;289(1):199–214. doi: 10.1111/febs.16112

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© 2021 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 2 — GATAD2B C terminus mediates interaction with CHD4. Volcano plots were generated using LFQ intensities of FLAG PD‐MS and the canonical subunits of the NuRD complex are highlighted in black and noncanonical or potential new interactors in grey. (A) Full‐length GATAD2B, (B) N terminus (GATAD2B‐N) and (C) C terminus (GATAD2B‐C). FLAG‐only was used as a control (CTRL) to account for background contamination. (D) FLAG PD‐MS of GATAD2B‐C^Del versus WT GATAD2B‐C. Schematic of GATAD2B constructs used as baits are indicated on top of the volcano plots. (E) Western blots of GATAD2B proteins co‐expressed in the IVT system and purified on anti‐FLAG beads. Input (5% of total, left panel) blot was developed with anti‐HA and the elution (70% of total, right panel) was developed with both HA and FLAG antibodies.