Fig. 5.

CHD3/4/5 C termini preferentially bind NuRD subunits and ADNP. The canonical subunits of the NuRD are highlighted in black, ADNP in red and potential new interactors in grey. Interaction partners of (A) CHD4‐N (B) CHD3‐C (C) CHD5‐C and (D) CHD4‐N compared to CTRL, and (E) CHD4‐N vs CHD4‐C (F) Network representation of shared and specific interactors of CHD proteins. Significantly enriched proteins with at least twofold change were used to generate the network. Bait proteins are indicated in blue circles, and unique interactors of each family member are indicated in dark orange and shared interactors in light orange. (G) ADNP copurifies with CHD4 and CBX3/HP1γ, known subunits of ChAHP complex. ZMYM family proteins are indicated in grey. (H) Western blots of input and elution samples from Flag‐ADNP‐N (1‐228 aa) co‐expressed with CHD4 protein. Left, pulldown showing that FLAG‐ADNP‐N purified on anti‐FLAG beads pulls down co‐expressed CHD4 full‐length (Lane 2) or CHD4‐C (Lane 3). Right, pulldown showing that CHD4 full‐length (Lane 2) or CHD4‐C (Lane 3) purified on anti‐HA beads pulls down co‐expressed FLAG‐ADNP‐N.