Fig. 7.

In Marchantia polymorpha inhibition of chloroplast translation by spectinomycin treatment or chloroplast stress imposed by norflurazon treatment does not result in GUN1‐mediated downregulation of the same set of genes as in seed plants. (a) Transcript levels of M. polymorpha orthologues of seed plant plastid retrograde signalling‐regulated genes LHCB1, LHCB2, RbcS, Beta‐CA (BCA), CHLI, CHLD, CHLH, CP12, FBPase, HEMA, HEMB, NR and PSBS in wild‐type and Mpgun1 mutant M. polymorpha spores germinated on control media or media supplemented with 500 μg ml⁻¹ spectinomycin. Transcript abundances are shown as log₂ fold‐difference compared to wild‐type M. polymorpha spores grown under control conditions. Each column represents an independent biological replicate transcriptome. (b–d) qPCR quantification of PhANG transcript levels in wild‐type and Mpgun1 mutant spores germinated on spectinomycin or norflurazon‐containing media. The transcript levels were normalized against MpEF1α and MpACT. Each dot represents an independent biological replicate, and bars illustrate the average of the three biological replicates. White asterisks indicate statistically significant differences between Mpgun1 and wild‐type for the same treatment (Tukey's HSD: *, P < 0.05; **, P < 0.01). The code and source data for reproducing (a) are obtainable from Dryad (10.5061/dryad.x0k6djhmk).