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. 2022 Sep 28;11:e76366. doi: 10.7554/eLife.76366

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© 2022, Chen and St Johnston

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 7. — (A) Canoe (red) localises to the enterocyte membranes (yellow arrows) that face the lumen above an integrating pre-enterocyte, and to the marginal zone above the newly formed septate junctions between the pre-enterocyte and neighbouring enterocytes. Sqh >UtrABD GFP labelling of Actin in green and α-spectrin in greyscale. (B) An enteroblast integrating between a canoe^R10 mutant enterocyte (green) and a heterozygous enterocyte. Canoe (red) is lost from the roof of the lumen on the side with the mutant enterocyte, but still marks the roof on the side with a non-mutant enterocyte (yellow arrow). (C) A canoe^R10 mutant pre-enterocyte (green) integrating between two heterozygous enterocytes. The PAC still forms normally in the absence of Canoe (red). α-spectrin in greyscale. (D–E) MARCM clones of canoe^jc homozygous mutant cells stained for Myo7a (red; D), α-spectrin (red; E) and Tsp2A (greyscale; E). The mutant cells form normal apical domains and septate junctions in the absence of Canoe. (F–G) MARCM clones of coracle^jc homozygous mutant cells stained for Tsp2a (red in F), α-spectrin (greyscale in F) and Actin (red in G). The mutant cells form normal apical domains and septate junctions in the absence of Coracle. * marks the homozygous mutant cells. Scale bar = 5 µm.

Figure 7—figure supplement 1. — (A) Canoe (red) localises to the enterocyte membranes (yellow arrows) that face the lumen above an integrating pre-enterocyte, and to the marginal zone above the newly formed septate junctions between the pre-enterocyte and neighbouring enterocytes. Sqh >UtrABD GFP labelling of Actin in green and α-spectrin in greyscale. (B) An enteroblast integrating between a canoe^R10 mutant enterocyte (green) and a heterozygous enterocyte. Canoe (red) is lost from the roof of the lumen on the side with the mutant enterocyte, but still marks the roof on the side with a non-mutant enterocyte (yellow arrow). (C) A canoe^R10 mutant pre-enterocyte (green) integrating between two heterozygous enterocytes. The PAC still forms normally in the absence of Canoe (red). α-spectrin in greyscale. (D–E) MARCM clones of canoe^jc homozygous mutant cells stained for Myo7a (red; D), α-spectrin (red; E) and Tsp2A (greyscale; E). The mutant cells form normal apical domains and septate junctions in the absence of Canoe. (F–G) MARCM clones of coracle^jc homozygous mutant cells stained for Tsp2a (red in F), α-spectrin (greyscale in F) and Actin (red in G). The mutant cells form normal apical domains and septate junctions in the absence of Coracle. * marks the homozygous mutant cells. Scale bar = 5 µm.