FIG. 2.

Western blot analysis of surface extracts from the Asc10 insertion mutants. The Western blot utilized a polyclonal antibody generated against the N terminus of Asc10. An equivalent amount of protein was added to each lane. The laddering pattern is typical of AS protein preparations, as they have high instability. Migration of molecular mass standard marker proteins is shown at the left of the blot, and the 137-kDa full-length Asc10 and 78-kDa fragment are indicated by arrows. 7517, Asc10⁺; 3535, vector control.