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Insertion mutants 2049 and 2421 have near-wild-type aggregation levels when grown at 30°C as indicated by spectrophotometry (A). Western blot analysis of surface extracts indicated much more reactive protein on these cells when they were grown at 30°C (B). Migration of molecular mass standard marker proteins is shown to the left of the blot, and the 137-kDa full-length and 78-kDa Asc10 fragments are indicated by arrows.
