TABLE 1.
Strains and plasmids used in this study
| Strain, phage, or plasmid | Strain or description | Source or reference |
|---|---|---|
| Bacteria | ||
| E. faecalis | CG1SSp | 6 |
| CG1RF | 6 | |
| E. coli | CC160 | C. Manoil |
| CC245 | C. Manoil | |
| CC118 | C. Manoil | |
| Phage | λTnlacZ/in | C. Manoil |
| λTnphoA/in | C. Manoil | |
| Plasmids | ||
| pWM402 | E. coli, E. faecalis shuttle vector | 37 |
| pGEX-4T | Expression–glutathione S-transferase fusion vector from the tac promoter | Pharmacia Biotech |
| pTRKH2 | Shuttle vector | 24 |
| pINY1801 | pCF10 positive control region from prgX through prgC | 5 |
| pMSP3601 | KpnI-EcoRI fragment of pTRKH2 blunt ended and inserted into BanII site of pINY1801 | This study |
| pMSP3602 | BamHI sites of pMSP3601 filled, first mutagenesis target | This study |
| pMSP3603.1 | BsrGI-BtrI PCR cloned into pGEXT-Easy | This study |
| pMSP3604 | BspHI-NcoI fragment of pMSP3603.1 inserted into XhoI-NcoI sites of pGEX-4T, making signal sequence deficient target | This study |
| pMSP7517 | Nisin-inducible prgB | 8 |
| pMSP7517ΔMscI | MscI fragment removed from pMSP7517 | This study |
| pMSP3535 | Nisin-inducible expression vector | 3 |
| pCF175 | Tn917 insertion into prgB of pCF10 | 5 |
| prgB insertions in pMSP7517a | ||
| pCWΩ96 | TnphoA/in insertion at base 96 | This study |
| pCWΩ258 | TnphoA/in insertion at base 258 | This study |
| pCWΩ324 | TnlacZ/in insertion at base 324 | This study |
| pCWΩ438 | TnlacZ/in insertion at base 438 | This study |
| pCWΩ468 | TnlacZ/in insertion at base 468 | This study |
| pCWΩ1074 | TnlacZ/in insertion at base 1074 | This study |
| pCWΩ1077 | TnlacZ/in insertion at base 1077 | This study |
| pCWΩ1299 | TnlacZ/in insertion at base 1299 | This study |
| pCWΩ1317 | TnlacZ/in insertion at base 1317 | This study |
| pCWΩ1419 | TnlacZ/in insertion at base 1419 | This study |
| pCWΩ1551 | TnlacZ/in insertion at base 1551 | This study |
| pCWΩ1638 | TnlacZ/in insertion at base 1638 | This study |
| pCWΩ2049 | TnlacZ/in insertion at base 2049 | This study |
| pCWΩ2064 | TnlacZ/in insertion at base 2064 | This study |
| pCWΩ2085 | TnlacZ/in insertion at base 2085 | This study |
| pCWΩ2421 | TnlacZ/in insertion at base 2421 | This study |
| pCWΩ2601 | TnlacZ/in insertion at base 2601 | This study |
| pCWΩ2760 | Reverse in-frame TnlacZ/in insertion at base 2760 | This study |
| pCWΩ2979 | TnlacZ/in insertion at base 2979 | This study |
| pCWΩ3102 | TnlacZ/in insertion at base 3102 | This study |
| pCWΩr3183 | Reverse in-frame TnlacZ/in insertion at base 3183 | This study |
| pCWΩ3414 | TnlacZ/in insertion at base 3414 | This study |
| pCWΩ3599 | Out-of-frame TnlacZ/in insertion at base 3599 resulting in a stop codon and no cell wall anchor | This study |
a
All have the BamHI fragment of the transposon removed.