Figure 4.

miR‐378a enhances interleukin (IL)‐17A‐mediated induction of nuclear factor kappa B (NF‐κB) activity in keratinocytes. (a) Immunofluorescence staining of p65 in keratinocytes overexpressing miR‐378a with or without treatment with IL‐17A (100 ng mL⁻¹) for 30 min; ‘mimic’ refers to miR‐378a mimic. Control mimic (scramble oligo) was used as a control. (b) Luciferase activity was measured in keratinocytes transfected with the NF‐κB reporter and miR‐378a mimic or control mimic for 24 h, alone or treated with IL‐17A (100 ng mL⁻¹) for 6 h. (c) The expression of CXCL8/IL‐8 and CCL20 were analysed by reverse‐transcription quantitative real‐time polymerase change reaction (RT‐qPCR) in cultured primary human keratinocytes upon transfection with miR‐378a mimic or mimic control, alone or in combination with IL‐17A (100 ng mL⁻¹). *P < 0·05, **P < 0·01, ***P < 0·001, ****P < 0·0001 (Student’s two‐sided t‐test). DAPI, 4′,6‐diamidino‐2‐phenylindole. [Colour figure can be viewed at wileyonlinelibrary.com]