Figure 5.

Inhibition of miR‐378a attenuates interleukin (IL)‐17A‐mediated induction of nuclear factor kappa B (NF‐κB) activity in keratinocytes. (a) Immunofluorescence staining of p65 in keratinocytes transfected with miR‐378a inhibitor, alone or in cotreatment with IL‐17A (100 ng mL⁻¹) for 30 min; ‘inhibitor’ refers to miR‐378a inhibitor. Control inhibitor (scramble oligo) was used as a control. (b) Luciferase activity of transfection with NF‐κB reporter and miR‐378a inhibitor or control inhibitor, alone or in cotreatment with IL‐17A (100 ng mL⁻¹). (c) RT‐qPCR analysis of the expression of CXCL8/IL‐8 and CCL20 in cultured primary human keratinocytes treated with miR‐378a inhibitor or inhibitor control, alone or in combination with IL‐17A (100 ng mL⁻¹). *P < 0·05, **P < 0·01, ***P < 0·001 (Student’s two‐sided t‐test). DAPI, 4′,6‐diamidino‐2‐phenylindole. [Colour figure can be viewed at wileyonlinelibrary.com]