TABLE 2.

Primer sequences used in this study

Primer name a	Primer sequence (excluding adapters) b	Amplicon size (excluding adapters)
mtCO1 gene‐specific primers
Wfly‐PCR‐F1	TGGTTYTTTGGTCATCCRGAAG	645 bp
Wfly‐PCR‐R1	GGAAARAAWGTTAARTTWACTCC
Wfly‐PCR‐F2	CGRGCTTAYTTYACTTCAGCYAC	663 bp
Wfly‐PCR‐R2	GGYTTATTRATTTTYCAYTCTA
ace1 gene‐specific primers
Bt‐ace‐F	TAGGGATCTGCGACTTCCC	287 bp
Bt‐ace‐R	GTTCAGCCAGTCCGTGTACT
vgsc gene‐specific primers
Bt‐kdr‐F1	GCCAAATCCTGGCCAACT	184 bp
Bt‐kdr‐Rintr1	GAGACAAAAGTCCTGTAGC

^a Specific primers for the partial amplification of mtCO1, ace1 and vgsc genes.

^b Given gene‐specific primer sequences are attached to the linker sequences (in bold) when ordering which Illumina adapters [i5] and [i7] are attached to the linker sequences during the second amplification step 5′‐[i5]TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG ‐3′ and 5′‐[i7]GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG ‐3′. ([i5] and [i7] are Nextera index sequences). Underlined sequences are recognition sites for trimming the adapter sequences during analysis steps (Illumina). Wfly‐PCR‐F1/R1 and Wfly‐PCR‐F2/R2 primers were the same as reported previously. ¹⁴