Ammonia released after incubation of biuret with cell
extracts from E. coli DH5α (atzE)
and E. coli DH5α (atzF). Symbols:
□, 3 mM biuret plus crude cell extracts from E.
coli DH5α (atzE) and E.
coli DH5α (atzF); ▿, 3 mM biuret plus cell
extract from E. coli DH5α (atzE);
▾, 3 mM urea plus cell extract from E. coli
DH5α (atzF); ○, 3 mM biuret plus cell extract
from E. coli DH5α (atzF); ●, 3
mM biuret plus cell extract from E. coli
DH5α(pUC18). Values are the means of results from three
replicates. Error bars indicate standard deviations of the means.