FIGURE 2.

Bronchial epithelial cells from patients with asthma were exposed to HDM or heat inactivated (hi)‐HDM (20 μg/ml) for 24 h, then stimulated with the viral mimic (Poly(I:C)) (10 μg/ml) for 24 h. 24 h gene expression of IL‐8 (A), TNF‐α (B), and β‐defensin‐2 (C) after Poly(I:C) (n = 20 except hi‐HDM n = 16). Protein levels of IL‐8 (D), TNF‐α (E), and β‐defensin‐2 (F) 24 h after Poly(I:C) (n = 7 except hi‐HDM n = 3). Cells were also divided into HDM negative (HDM‐; n = 13) and HDM positive (HDM+; n = 7) cells (G‐I) with 24 h gene expression of IL‐8 (G), TNF‐α (H), and β‐defensin‐2 (I). Samples were analyzed by the ΔCt method and normalized to UBC/GAPDH expression. Data are presented as median (interquartile range)