TABLE 1.

Tick‐borne pathogens investigated in this study with target genes, primers nucleotide sequences and fragment length

Tick‐borne pathogens	Target gene	Primers	Sequence (5′−3′)	Fragment length (bp † )	References
Anaplasma/Ehrlichia spp. Borrelia burgdorferi sensu lato complex Coxiella burnetii Rickettsia spp. Spotted fever group rickettsiae	16S rRNA Flagellin IS1111a gltA ompA	EHR‐16SD EHR‐16SR FLA1 FLA2 Trans‐1 Trans‐2 CS‐78F CS‐323R Rr190.70F Rr190.701R	GGTACCYACAGAAGAAGTCC TAGCACTCATCGTTTACAGC AGAGCAACTTACAGACGAAATTAAT CAAGTCTATTTTGGAAAGCACCTAA TATGTATCCACCGTAGCCAGT CCCAACAACACCTCCTTATTC GCAAGTATCGGTGAGGATGTAAT GCTTCCTTAAAATTCAATAAATCAGGAT ATGGCGAATATTTCTCCAAAA GTTCCGTTAATGGCAGCATCT	345 482 687 401 632	Martin et al. (2005) Wójcik‐Fatla et al. (2009) Berri et al. (2000) Labruna et al. (2004) Regnery et al. (1991)

^† bp = base pairs.