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The atf1 mutant GPLs are not acetylated. GPLs analyzed by TLC are shown for the indicated strains. Lipid extracts were made from cells grown on plates and either subjected to mild NaOH deacetylation treatment (+) or not deacetylated (−). The dots indicate the GPLs observed by the Brennan and Goren method of GPL analysis that includes deacetylation. WT, wild type.
